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KMID : 0363420040180010011
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Journal of Korean Oriental Pediatrics
2004 Volume.18 No. 1 p.11 ~ p.25
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Effect of velvet antler on the function of adipocytes(3T3-L1) and its association with IGF-1
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Lee Jin-Yong
Kim Deog-Gon
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Abstract
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Objectives: These experimental studies were designed to investigate the effects of antler’s extracts on the expression of leptin, IGF-l and IGF-1 receptor on cultured 3T3-L1 cells.
Methods: A mouse adipoblast cell line, 3T3-L1, was cultured with or without a differentiation medium containing Isobutylmethylxanthin, Dexametasone and Insulin before adding extracts of antler of various concentratio(10, 50, ]00, 200${\mu}g/ml$). The expression of leptin and IGF-1 receptor was measured by western blot assay, and expression of IGF-1 was determined by F ACS analysis.
Result and Conclusion: The 3T3-L1 cells’’ differentiation did not show a significant induction by extracts of antler. The expression of leptin was significantly decreased depend on antler’s concentration. The expression of IGF-1 showed a slightly increase by extracts of antler, whereas that of IGF-receptor showed a tendency to increase. The total amount of intracellular triglyceride showed a tendency to diminish as the concentration of antler’s extract increase.
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KEYWORD
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velvet antler, leptin, IGF_1, IGF-1 receptor, 3T3-L1 cells
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